Description

Illuminate transcription and translation with the BioBits® cell-free system in this innovative classroom-sized kit! 

BioBits® pellets are tiny molecular factories that can create proteins without the need for cell culture. When dry, BioBits® pellets are dormant, but they can be activated by simply adding water. Researchers have been using cell-free reactions in their laboratories for years, with applications ranging from novel therapeutic discovery to field diagnostics. Now the BioBits® cell-free system makes this cutting-edge technology accessible to anyone interested in learning molecular biology. With minimal equipment requirements and a quick and straightforward protocol, students will use the BioBits® system to visualize the flow of genetic information and monitor transcription and translation in real-time through fluorescence. This activity serves as an excellent interactive tool for learning molecular biology’s central dogma and exposes students to cutting-edge synthetic biology.

• Techniques: Micropipetting, fluorescence detection, cell-free technology
• Topics: Central dogma of molecular biology, transcription and translation, protein synthesis, gene expression, fluorescence, biotechnology, synthetic biology
• Time required: 30 minutes during 1 class period, followed by 10 minutes for observation 8-72 hours later
• Level: General high school through college

The Central Dogma Lab curriculum is now available on miniPCR Digital. Purchase access to the Central Dogma Digital Lab to replace your printed lab manual with an interactive lab experience.

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