Gel Loading Dye, Blue (10X) is a pre-mixed loading buffer with one tracking dye for agarose and non-denaturing poylacrylamide gel electrophoresis. EDTA is included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Bromophenol blue is the standard tracking dye for electrophoresis.
Gel Loading Dye, Blue (10X) is assayed for contaminating endonuclease, exonuclease and RNase activity.
10 mM Tris-HCl（pH 7.6）
0.03% Bromophenol Blue
60 mM EDTA
Bromophenol Blue migrates at approximately 370 bp on a standard 1% TAE agarose gel, and 220 bp on a standard 1% TBE agarose gel.
Storage: Room temperature