Description
Illuminate transcription and translation with the BioBits® cell-free system.
BioBits® pellets are tiny molecular factories that can create a variety of proteins, from brightly colored fluorescent proteins to functional enzymes, without the need for cell culture. When dry, BioBits® pellets are dormant, but they can be activated by simply adding water. Researchers have been using cell-free reactions in their laboratories for years, with applications ranging from novel therapeutic discovery to field diagnostics. Now the BioBits® cell-free system makes this cutting-edge technology accessible anywhere to anyone interested in learning molecular biology and is an excellent teaching tool to enhance biology education both within and beyond the classroom.
With minimal equipment requirements and a quick and straightforward protocol, students will use the BioBits® system to visualize the flow of genetic information and monitor transcription and translation in real time through fluorescence. This activity serves as an excellent interactive tool for learning the central dogma of molecular biology and exposes students to cutting-edge synthetic biology.
- Techniques utilized: Micropipetting, cell-free protein synthesis
- Time required: 30 min + 10 min periods, separated by 8 to 72 hours
Level: Introductory to advanced.
The BioBits™ Central Dogma kit contains the following reagents:
- 32 BioBits® pellets in 8-tube PCR strips
- DNA Sample A
- DNA Sample B
- Nuclease-Free Water
- Kanamycin
Required equipment:
- P51™ Molecular Fluorescence Viewer or blue light transilluminator (460-480 nm, e.g. blueBox or blueGel) — one per lab group
- Note: if you have a P51™ viewer purchased before September 2019,contact us to request an orange filter with your BioBits order
Storage notes:
- These reagents are best stored in a freezer or fridge.
- Use within 6 months of receipt when stored in a freezer (-20ºC).
- If a freezer is not available, BioBits® pellets are viable for three months when stored in a fridge (4ºC ).
Product documentation:
Free miniPCR Learning Lab™ downloads:
- BioBits® Central Dogma Lab Instructor’s Guide
- BioBits® Central Dogma Lab Student’s Guide
- BioBits® Central Dogma Classroom Slides
- BioBits® Central Dogma Lab Live Demo (YouTube)
Contact biobits@minipcr.com for answers key.
