Publications
Research enabled by miniPCR® technology
- A fluorescence viewer for rapid molecular assay readout in space and low-resource terrestrial environments. Misquitta et al., PLoS One (2024). https://doi.org/10.1371/journal.pone.0291158
- A PCR Test Using the Mini-PCR Platform and Simplified Product Detection Methods Is Highly Sensitive and Specific to Detect Fasciola hepatica DNA Mixed in Human Stool, Snail Tissue, and Water DNA Specimens. Fernandez-Baca et al., Pathogens (2024). https://doi.org/10.3390/pathogens13060440
- Field-based molecular detection of Batrachochytrium dendrobatidis in critically endangered Atelopus toads and aquatic habitats in Ecuador. Riascos-Flores et al., PLOS ONE (2024). https://doi.org/10.1371/journal.pone.0299246
- Open Educational Resources for distributed hands-on teaching in molecular biology. Cerda et al., bioRxiv (2024). https://doi.org/10.1101/2024.03.28.587173
- Validation of Cell-Free Protein Synthesis Aboard the International Space Station. Kocalar et al., ACS Synth. Biol. (2024). https://doi.org/10.1021/acssynbio.3c00733
- MiniPCR as a portable equipment for the molecular diagnosis of american cutaneous leishmaniasis. Castellanos-Gonzalez et al., Acta Trop. (2023). https://doi.org/10.1016/j.actatropica.2023.106926
- Detecting Mediterranean White Sharks with Environmental DNA. Jenrette, J.F., et al. Oceanography (2023). https://doi.org/10.5670/oceanog.2023.s1.28
- Combination of ultra-rapid DNA purification (PURE) and loop-mediated isothermal amplification (LAMP) for rapid detection of Trypanosoma cruzi DNA in dried blood spots. Longhi et al., PLOS Neg. Trop. Dis. (2023). https://doi.org/10.1371/journal.pntd.0011290
- DNA storage in thermoresponsive microcapsules for repeated random multiplexed data access. Nat. Nanotechnol. (2023). https://doi.org/10.1038/s41565-023-01377-4
- PNA-Pdx: Versatile Peptide Nucleic Acid-Based Detection of Nucleic Acids and SNPs. Jiang et al., Anal. Chem. (2023). https://doi.org/10.1021/acs.analchem.3c01809
- DNA barcoding of sea urchin species in the bird’s head seascape Papua-Indonesia. Dailami et al., IOP Conf. Ser.: Earth Environ. Sci. (2023). DOI 10.1088/1755-1315/1191/1/012002
- Before you go: a packing list for portable DNA sequencing of microbiomes and metagenomes. Edwards et al., Microbiology Research (2022). https://doi.org/10.1099/mic.0.001220
- Two large structure-forming sponges from opposite North American coasts: a taxonomic review of Arctic–Pacific Mycale (Mycale) loveni and the description of a new Arctic–Atlantic Mycale. Dinn et al., Canadian Journal of Zoology (2023). https://doi.org/10.1139/cjz-2023-0011.
- Environmental DNA and visual encounter surveys for amphibian biomonitoring in aquatic environments of the Ecuadorian Amazon. Quilumbaquin W, et al., Aquatic Biology (2023). https://peerj.com/articles/15455/
- Laboratory evaluation of the miniature direct-on-blood PCR nucleic acid lateral flow immunoassay (mini-dbPCR-NALFIA), a simplified molecular diagnostic test for Plasmodium. van Dijk NJ, et al., Malar J (2023). doi: 10.1186/s12936-023-04496-4.
- Portable nucleic acid tests for rapid detection of monkeypox virus. Bhadra and Ellington, MedRxiv (2022). https://doi.org/10.1101/2022.08.09.22278605
- Towards an in situ non-lethal rapid test to accurately detect the presence of the nematode parasite, Anguillicoloides crassus, in European eel, Anguilla anguilla. De Noia et al., Parasitology (2022). https://doi.org/10.1017/s0031182021002146
- Rapid in situ identification of biological specimens via DNA amplicon sequencing using miniaturized laboratory equipment. Pomerantz et al., Nature Protocols (2022). https://doi.org/10.1038/s41596-022-00682-x
- CREST, a Cas13-Based, Rugged, Equitable, Scalable Testing (CREST) for SARS-CoV-2 Detection in Patient Samples. Aralis et al., Current Protocols, (2022). https://doi.org/10.1002/cpz1.385
- A miniPCR-Duplex Lateral Flow Dipstick Platform for Rapid and Visual Diagnosis of Lymphatic Filariae Infection. Phuakrod et al., Diagnostics (Basel), (2021).
- A Scalable, Easy-to-Deploy, Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material. Rauch et al., JCM, (2021).
- Detection of SARS-CoV-2 RNA using RT-LAMP and molecular beacons. Sherrill-Mix et al., Genome Biology, (2021).
- A Novel Miniature CRISPR-Cas13 System for SARS-CoV‑2 Diagnostics. ACS Synth. Biol. (2021), 10, 2541−2551.
- Morphological stasis masks ecologically divergent coral species on tropical reefs. Bongaerts et al., Curr. Bio. (2021)
- Portable PCR field-based detection of sweetpotato viruses. Ssengo et al., African Crop Science Journal (2021). DOI: 10.4314/acsj.v28i3.3
- A CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station. Stahl-Rommel et al., PLOS ONE (2021).
- Real-Time Culture-Independent Microbial Profiling Onboard the International Space Station Using Nanopore Sequencing. Stahl-Rommel et al., Genes (2021).
- Stony Coral Tissue Loss Disease biomarker bacteria identified in corals and overlying 2 waters using a rapid field-based sequencing approach. Becker et al., BioRxiv (2021).
- Prevalence of integrons in Enterobacteriaceae obtained from clinical samples. Barns et al., Journal of Microbiology and Antimicrobials (2021).
- In search of the RNA world on Mars. Mojarro et al., Geobiology (2021).
- Simpler and faster COVID-19 testing: Strategies to streamline SARS-CoV-2 molecular assays. Panpradista et al., EBioMedicine (2021).
- Multiplex Isothermal Amplification Coupled with Nanopore Sequencing for Rapid Detection and 2 Mutation Surveillance of SARS-CoV-2. Bi et al., MedRxiv (2021).
- Scaling diagnostics in times of COVID-19: Colorimetric Loop-mediated Isothermal Amplification (LAMP) assisted by a 3D-printed incubator for cost-effective and scalable detection of SARS-CoV-2. González-González et al., PLOS ONE (2020).
- Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids. Attoye et al., Biosensors (2020).
- Portable Rabies Virus Sequencing in Canine Rabies Endemic Countries Using the Oxford Nanopore MinION. Gigante et al., Viruses (2020).
- Portable and accurate diagnostics for COVID-19: Combined use of the miniPCR thermocycler and a well-plate reader for SARS-Co2 virus detection. Gonzalez-Gonzalez et al., MedRxiv (2020).
- Influenza A Virus Field Surveillance at a Swine-Human Interface. Rambo-Martin et al., Clinical Science and Epidemiology (2020).
- One‐Step Nucleic Acid Purification and Noise‐Resistant Polymerase Chain Reaction by Electrokinetic Concentration for Ultralow‐Abundance Nucleic Acid Detection. Ouyang and Han, Angewandte Chemie (2020).
- Field-Adapted Full Genome Sequencing of Peste-Des-Petits-Ruminants Virus Using Nanopore Sequencing. Torsson et al., Front Vet Sci. (2020).
- Metagenomic water quality monitoring with a portable laboratory. Acharya et al., Water Res. (2020).
- Next-generation in situ conservation and capacity building in Madagascar using a mobile genetics lab. Blanco et al., BioRxiv (2019).
- Nucleic acid detection aboard the International Space Station by colorimetric loop‐mediated isothermal amplification (LAMP) (Rubinfien et al., FASEB BioAdvances, 2019).
- Validation of use of the miniPCR thermocycler for Ebola and Zika virus detection. Gonzalez-Gonzalez et al. PLoS One (2019).
- Backpack PCR: A point-of-collection diagnostic platform for the rapid detection of Brugia parasites in mosquitoes. Zaky et al., PLOS Neglected Tropical Diseases (2018).
- PCR: Thirty-five years and counting. (Science Magazine Technology Feature mentioning miniPCR.)
- Successful Amplification of DNA aboard the International Space Station. Boguraev et al., NPJ Microgravity (2017).
- Gene expression studies using a miniaturized thermal cycler system on board the International Space Station. Montague et al., PLOS ONE (2018).
- Real-time DNA barcoding in a rainforest using nanopore sequencing: opportunities for rapid biodiversity assessments and local capacity building. Pomerantz et al., GigaScience, giy033, (2018). PDF
- Rapid identification of infections. Park et al., Science Advances (2016).
- A simple, economical protocol for DNA extraction and amplification where there is no lab. Guevara et al., 2017. PDF
- Performance of miniPCRTM mini8, a portable thermal cycler. Kwon et al., 2016.
- PCR Heads into the field. Technology feature, Nature Methods 2015, Vol. 12 No. 5